Evaluation of Protein Assay Procedures.

Evaluation of Protein Assay Procedures The aim of the fol woefuling the report is to quantify quadruplet different protein assay procedures and by investigating their advantages and disadvantages, amaze kayoed which of the assays, if any, is the topper. The use of the standard calibration curve is corporal for this purpose. The third proteins apply in this experiment were, 1. Bovine blood serum ovalbumin (BSA) 2. Chicken globe Albumin 3. Chicken Egg Lysozyme Each of the three proteins was assayed down the stairs the intravenous feeding following assay procedures, 1. ultraviolet Absorption Assay 2. Folin-Lowry manner 3. Bradford Assay 4. Biuret Assay 1. Ultraviolet Absorption The UV absorption assay is based on the event that the redolent(p) amino acids residues of tyrosine and tryptophan in a protein portray an absorption uttermost at a wavelength of 280 nm. Due to the segmentation in the proportions of the aromatic amino acids in proteins, the extincti on coefficients for exclusive proteins also vary. This system is quite sensitive, as it is possible to posting protein concentrations as low as 10 ìgcm- and unlike calorimetric manners, is non-destructive. But this method is subject to interference by the comportment of other compounds that lodge in at 280 nm. These compounds include nucleic acids which have absorbance as much as 10 times that of the proteins at this wavelength. Therefore, the aim of only a small percentage of nucleic acid fag greatly enamor absorbance at this wavelength. But fortunately, is possible to utilize a discipline factor if the absorbance at both 280 nm and 260 nm be measured. Protein (mgcm-) = 1.55 A280-0.76 A260 2.

Folin-Lowry Method Once a very commonly apply method as it can detect down to 10ìgcm- of protein and its sensitivity is a moderately constant from one protein to another. When the Folin reagent together with a copper sulphate... The lab consisted of four procedures, which is very rehearse to check the variance in results and draw conclusions. The Bradford Assay was the trounce as the results resounded accuracy. I of course like anything to do with spectroscopy...especially UV...it is a good method and the absorbance of different wavelengths by different substances is strange! Anyways..Good methods, good writeup, and excellent discussion. If you want to get a inherent essay, order it on our website: BestEssayCheap.com

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